Measurement of Urinary .DELTA.-Aminolevulinic Acid (ALA) by Fluorometric HPLC and Colorimetric Methods.
نویسندگان
چکیده
منابع مشابه
Delta-aminolevulinic acid dehydratase (ALAD) polymorphism in lead exposed Bangladeshi children and its effect on urinary aminolevulinic acid (ALA).
BACKGROUND AND OBJECTIVE Lead has long been recognized as a harmful environmental pollutant. People in developing countries like Bangladesh still have a higher risk of lead exposure. Previous research has suggested that the delta-aminolevulinic acid dehydratase (ALAD) genotype can modify lead toxicity and individual susceptibility. As children are more susceptible to lead-induced toxicity, this...
متن کاملInterrelation between urinary delta-aminolevulinic acid (ALA), serum ALA, and blood lead in workers exposed to lead.
Using a fluorometric HPLC method, we determined delta-aminolevulinic acid (ALA) in sera and urine samples from 16 lead workers with blood lead levels ranging from 19 to 107 microG/100 ml. The concentration of ALA in serum correlated highly with the urinary ALA concentration (gamma = 0.957 for ALA mg/1; gamma = 0.967 for ALA mg/g creatinine). The ALA concentrations in the serum of lead workers r...
متن کاملOptimized fluorometric determination of urinary delta-aminolevulinic acid by using pre-column derivatization, and identification of the derivative.
We previously reported the usefulness of a fluorometric method to determine urinary delta-aminolevulinic acid (ALA) concentrations by using post-column derivatization to monitor the effect of lead exposure. We have further improved the method by introducing pre-column derivatization by using reaction of ALA with acetylacetone and formaldehyde. Response of the hematopoietic system to lead exposu...
متن کاملOptimized liquid-chromatographic method for fluorometric determination of urinary delta-aminolevulinic acid in workers exposed to lead.
We developed a fluorometric method for determining delta-aminolevulinic acid (ALA) in urine of lead workers. A high-performance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 micrograms/L (15 pmol of ALA in the 100-microL sample). The working linear range of urinary ALA concentration was 0.1 to 100 mg/L. In 25 lead-expose...
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Submitters: Charles R. Ratliff and Frank F. Hall, Section of Biochemistr’.,’, Scott & White Clinic; and Scott & White Hospital Foundation, Temple, Tex. 76501 Evaluators: Thomas W. CuIp, BioRegional Reference Laboratory, Medical Arts Building, San Antonio, Tex. 78205 Christopher S. Frings, Medical Laboratory Associates, 1Q25 South 18th St., Birmingham. Ala. 3520.5 James L. Gilleland and John E. ...
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ژورنال
عنوان ژورنال: INDUSTRIAL HEALTH
سال: 1992
ISSN: 0019-8366,1880-8026
DOI: 10.2486/indhealth.30.119